LOCUS       40924_44814       12063 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pUB-GW-Hyg DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12063)
  AUTHORS   Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, 
            Hayashi M.
  TITLE     Polyubiquitin Promoter-Based Binary Vectors for Overexpression and 
            Gene Silencing in Lotus japonicus
  JOURNAL   Mol. Plant Microbe Interact. 21 (4), 375-382 (2008)
  PUBMED    18321183
REFERENCE   2  (bases 1 to 12063)
  AUTHORS   Maekawa T, Hayashi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2007) Contact:Makoto Hayashi National Institute of
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan
REFERENCE   3  (bases 1 to 12063)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12063)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant 
            Microbe Interact."; date: "2008"; volume: "21"; issue: "4"; pages: 
            "375-382"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2007) Contact:Makoto Hayashi National Institute of 
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(103..277)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(320..1342)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(1416..1760)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    2278..2299
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2314..2344
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2352..2368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2376..2392
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2414..2666)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2694..2818
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2862..3164)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(3509..4165)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4219..4249)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(4274..4398)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4402..4418)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(4419..5005)
                     /gene="Ljubq1"
                     /label=Ljubq1
     intron          complement(4419..4958)
                     /gene="Ljubq1"
                     /note="5' UTR intron of Ljubq1 gene"
     exon            complement(4959..5005)
                     /gene="Ljubq1"
                     /number=1
     regulatory      complement(5006..5540)
                     /label=Ljubq1 promoter
                     /note="Ljubq1 promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(5567..5583)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5786..5810
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7110..7736
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8173..9237
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9306..9500
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9844..9984
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10170..10758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10848..11639)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat
      301 ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac
      361 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
      421 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
      481 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
      541 ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt
      601 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
      661 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
      721 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
      781 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
      841 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
      901 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
      961 taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca
     1021 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
     1081 tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
     1141 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
     1201 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
     1261 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
     1321 cggtgagttc aggctttttc atatctcatt gccccccggg atctgcgaaa gctcgagaga
     1381 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
     1441 ccttatatag aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
     1501 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     1561 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     1621 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     1681 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     1741 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     1801 tggagtagac gagagtgtcg tgctccacca tgttatcaca tcaatccact tgctttgaag
     1861 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     1921 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     1981 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     2041 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     2101 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     2161 tggcaagctg ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2221 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2281 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2341 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2401 cgaattcgag ctcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
     2461 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
     2521 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
     2581 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
     2641 ctttattgcc aaatgtttga acgatcgatg gcgcgcctag gtaccggatc atcaccactt
     2701 tgtacaagaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     2761 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggt
     2821 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg
     2881 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
     2941 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
     3001 tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg
     3061 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga
     3121 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt
     3181 ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg
     3241 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct
     3301 taccagaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca
     3361 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca
     3421 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg
     3481 cttttagtaa gccggatcca cgcgtttacg ccccgccctg ccactcatcg cagtactgtt
     3541 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     3601 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     3661 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     3721 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     3781 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     3841 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     3901 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     3961 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     4021 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     4081 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     4141 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat
     4201 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
     4261 ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
     4321 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
     4381 ttttttgtac aaacttgtga tgatccacta gttctagact gtaatcacat caacaacaga
     4441 taaatcagaa tccaacacac gaaattgaaa caagtgaaaa ccctaattaa cgcttggatc
     4501 aaacagatta agagtggatc taacatcaac agataaatca gaatccataa caagaaattg
     4561 aaacaagtga aaaccctaat tgaagcttgg atcaaacaga ttaagagtgg atctaacaat
     4621 aatttccaat ttgaataaat tatttgacat aattccataa cggatctgaa gaattcaatt
     4681 ggaaacccta attaatctgc aattgctaaa ccgaaatcga ttcctaacaa aactgaacca
     4741 caattatcat agatctacat cgcaagccat cgaaaacatt gataaaacaa gatctgagat
     4801 tcaagctgta aaattcaatt tcaaaccgat ctcaattttg gaaaatcaca aaaaattcct
     4861 agatctcatg cttcgaaaca agaaaactac ctatgattga tcggaaacaa aactcaacga
     4921 aaggattaca gagaaagaag agagaaattg gatcatacct tgaaagtaga gagaagcgat
     4981 atgaacaaga attgttaggg ttgggaactg aattgtgaag aagctaaggg gattggaggt
     5041 aatttatagc aaaagcggtg aaattattaa ccagtcaaat tgtgacacgt gtccttatga
     5101 cgcgtcacta gttgcgtgtg gtttgggata ggatacgttg tgtggttgag ttcttcttgg
     5161 aaacggtagt tgcaaattgc aagttgttgg cggtttgtta atttcacgga gttaaacgca
     5221 tctcgccgcc ttttgagttt ggattgataa atcgttacaa cggcgttact ttgctgggcc
     5281 ccgttttttt tgaattgggc caccgcacca gtgcaagttg ggggttcctt ggttttccag
     5341 aatgggctcc actctctatg attgattgga ggaggccggc tgctcagaaa ttttataata
     5401 agttgttata tacttctccc cttaaaataa gtgatttttg tctatccata gttgcaataa
     5461 gttgtactaa caacttatct aaattacttt atcaataata caagtaattc aattaattat
     5521 ttcctcaaaa tcctctctcc atcgaattcc tgcagcccag cttggcactg gccgtcgttt
     5581 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5641 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5701 tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc
     5761 gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     5821 agagcgttta ttagaataac ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     5881 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     5941 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga
     6001 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     6061 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     6121 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     6181 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     6241 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     6301 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     6361 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     6421 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     6481 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     6541 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     6601 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     6661 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     6721 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     6781 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     6841 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     6901 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     6961 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     7021 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     7081 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     7141 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     7201 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     7261 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     7321 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     7381 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     7441 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     7501 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     7561 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     7621 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     7681 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     7741 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     7801 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
     7861 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
     7921 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
     7981 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
     8041 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
     8101 gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg
     8161 aatcggcgtg acggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat
     8221 gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa
     8281 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa
     8341 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat
     8401 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg
     8461 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt
     8521 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac
     8581 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg
     8641 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc
     8701 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac
     8761 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta
     8821 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg
     8881 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac
     8941 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt
     9001 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg
     9061 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag
     9121 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc
     9181 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa
     9241 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc
     9301 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg
     9361 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata
     9421 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa
     9481 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg
     9541 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc
     9601 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc
     9661 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg
     9721 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     9781 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     9841 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     9901 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     9961 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
    10021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    10081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    10141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    10201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10501 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10561 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    10621 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10681 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10741 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10801 aacgaaaact cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc
    10861 agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat
    10921 agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg
    10981 tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca
    11041 tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg
    11101 ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct
    11161 tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct
    11221 aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc
    11281 ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct
    11341 tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt
    11401 tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt
    11461 tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg
    11521 ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt
    11581 acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat tttagccatt
    11641 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
    11701 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
    11761 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
    11821 cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
    11881 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
    11941 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
    12001 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
    12061 tgg
//
